Description:

Recombinant Tobacco Etch Virus protease (rTEV) is a recombinant expression in E. coli, with DsbA fusion tags cysteine protease. TEV recognizes the amino acid sequence of the general form Glu - Asn - Leu - Tyr - Phe - Gln - Gly/Ser, and cleaves between Gln and Gly/Ser, between amino acid residues can be cut with the identification of sequence of fusion protein.

Source: E. coli.

Molecular Mass: Approximately 51.5kD.

Purity: ≥95%.

Endotoxin Level: <1EU/µg, determined by the LAL method.

Unit Definition:

One unit is defined as the amount of enzyme needed to cleave 3 μg of fusion protein in 1 hour to 85 % completion at 30°C in a buffer containing 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, and 1 mM DTT.

Formulation:

50mM PBNa, pH7.4, 50 mM NaCl,5mM DTT,1mM EDTA, 50% glycerol.

Storage:

• rTEV in 50mM PBNa, pH7.4, 50 mM NaCl, 5mM DTT,1mM EDTA, 50% glycerol should be stored at -20℃.  It would be stable for 3 years when stored properly.

For Laboratory Use Only.