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    Home / Product Category / Reagents / Interleukins / Human Interleukin 18 (rHuIL-18)
    Human Interleukin 18 (rHuIL-18)

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    Human Interleukin 18 (rHuIL-18) Human Interleukin 18 (rHuIL-18)

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    Human Interleukin 18 (rHuIL-18)

    Availability:
    Quantity:
     
     
    • SJB06

    • 25μg

    Specifications and Use

    Description:

    Recombinant human IL-18 produced in yeast is a polypeptide chain containing 157 amino acids, with 4 Cys, but no disulfide bond.

    Source: Yeast.

    Molecular Mass: Approximately 18.2kD.

    Purity: ≥97%.

    Endotoxin Level: <1EU/µg, determined by the LAL method.

    Biological Activity:

    Induction of IFN-γ by KG-1 cell in response to the recombinant human IL-18 is measured using human IFN-γ ELISA.

    The activity should be as follow:

    IL-18 concentration (ng/ml)   IFN-γ induction (IU/ml)

          80                                               ≥15

          40                                               ≥10

    IFN-γ producing activity of the sample cells can be varied depends on cell conditions. Optimal concentration for each application should be determined by each laboratory.

    Formulation:

    Lyophilized from a 0.2μm filtered solution in PBS containing 0.2% HSA, pH7.4

    Reconstitution:

    It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.

    Storage:

    • Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.

    • Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

    • Avoid repeated freeze-thaw cycles.


    For Laboratory Use Only.


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