Description:

Recombinant human IL-18 produced in yeast is a polypeptide chain containing 157 amino acids, with 4 Cys, but no disulfide bond.

Source: Yeast.

Molecular Mass: Approximately 18.2kD.

Purity: ≥97%.

Endotoxin Level: <1EU/µg, determined by the LAL method.

Biological Activity:

Induction of IFN-γ by KG-1 cell in response to the recombinant human IL-18 is measured using human IFN-γ ELISA.

The activity should be as follow:

IL-18 concentration (ng/ml)   IFN-γ induction (IU/ml)

      80                                               ≥15

      40                                               ≥10

IFN-γ producing activity of the sample cells can be varied depends on cell conditions. Optimal concentration for each application should be determined by each laboratory.

Formulation:

Lyophilized from a 0.2μm filtered solution in PBS containing 0.2% HSA, pH7.4

Reconstitution:

It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.

Storage:

• Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.

• Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

• Avoid repeated freeze-thaw cycles.

For Laboratory Use Only.