Description:

Recombinant human GM-CSF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids, two pairs of disulfide bonds and having a molecular mass of approximately 14.6kD.

Source: E. coli.

Molecular Mass: Approximately 14.6kD.

Purity: ≥97%.

Endotoxin Level: <1EU/µg, determined by the LAL method.

Biological Activity:

Measured in a cell proliferation assay using the factor-dependent cell line, TF-1, the specific activity shall be not less than 1×10⁷IU/mg.

Formulation:

Lyophilized from a 0.2μm filtered solution in 10mM Phosphate Buffer.

Reconstitution:

It is recommended to centrifuge at 10,000rpm for 30-60 sec or 3000rpm for 5 min before opening the tube, and then reconstitute with 0.2ml sterile water.

Storage:

• Lyophilized samples are stable for 36 months from date of manufacture at -20℃ to -70℃.

• Upon reconstitution, this cytokine can be stored under sterile conditions at 2- 8℃ for one month or at -20℃ to -70℃ in a manual defrost freezer for three months without detectable loss of activity.

• Avoid repeated freeze-thaw cycles.

For Laboratory Use Only.